|Product name：||ATG5 (3C7) Mouse mAb|
|Recommended dilutions：||WB 1:5,000-10,000 IHC 1:100-200|
|Storage：||PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.|
|Human Swiss-Prot No.：||Q9H1Y0|
|Cellular localization：||Cytoplasm, Membrane|
|Alternative Names：||APG5, APG5L, ASP, Autophagy protein 5 antibody|
|Background：||Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.|
Immunohistochemical analysis of paraffin-embedded Human Ovarian Carcinoma Tissue using ATG5 （EM1374）Mouse mAb diluted at 1:200.
Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using ATG5 （EM1374）Mouse mAb diluted at 1:200.
Western blot analysis of Hela Cell Lysate using ATG5 （EM1374）Mouse mAb diluted at 1:10000.