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        Histone H3 (tri methyl K79) Monoclonal antibody

        Catalog No. : EM1091

        规格 价格
        50µl ¥1280
        100µl ¥1980

        Overview

        Product name: Histone H3 (tri methyl K79) Monoclonal antibody
        Source: Mouse
        Applications: WB, IHC
        Species reactivity: Human,Rat,Mouse
        Recommended dilutions: WB 1:500-2,000 IP 1:200  IHC 1:200-500
        Immunogen: Synthetic Peptide
        Specificity: Human
        Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
        Clonality: Monoclonal
        Isotype: IgG1
        Concentration: 1mg/ml
        Observed band: 15kDa
        GeneID(Human): 8290
        Human Swiss-Prot No.: P68431
        Cellular localization: Nucleus,Chromosome
        Alternative Names: H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody
        Background: Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
        • Western blot analysis of Hela with Histone H3 (tri methyl K79) Mouse mAb(3G3) diluted at 1)1:2,000 2)1:5,000

        • Western blot analysis of 1) Hela, 2) Rat Testis tissue, 3) Raw264.7 with Histone H3 (tri methyl K79) (3G3) Mouse MAb diluted at 1:2000.

        • 1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1091 1:1,000 Secondary antibody: Goat anti-Mouse IgG(H+L) (S001) 1:10,000

        • Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using  Histone H3(tri methyl K79)(EM1091)Mouse mAb diluted at 1:500.