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      Histone H3(di methyl K27) Monoclonal antibody

      Catalog No. : EM1101

      规格 价格
      50µl ¥1280
      100µl ¥1980

      Overview

      Product name: Histone H3(di methyl K27) Monoclonal antibody
      Source: Mouse
      Applications: WB, IHC
      Species reactivity: Human,Rat,Mouse
      Recommended dilutions: WB 1:1,000-3,000 IP 1:200  IHC 1:200-500
      Immunogen: Synthetic Peptide
      Specificity: Human
      Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
      Clonality: Monoclonal
      Isotype: IgG1
      Concentration: 1mg/ml
      Observed band: 15kDa
      GeneID(Human): 8290
      Human Swiss-Prot No.: P68431
      Cellular localization: Nucleus,Chromosome
      Alternative Names: H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody
      Background: Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
      • Western blot analysis of Hela withHistone H3 (di methyl K27) (1E10) Mouse MAb diluted at 1) 1:1,000 2) 1:3,000

      • 1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1101 1:2,000 Secondary antibody: Goat anti-Mouse IgG(H+L) (S001) 1:10,000

      • Immunohistochemical analysis of paraffin-embedded Human Skin using  Histone H3(di methyl K27)(EM1101)Mouse mAb diluted at 1:500.