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          Histone H3(tri methyl K9) Monoclonal antibody

          Catalog No. : EM1109

          规格 价格
          50µl ¥1280
          100µl ¥1980


          Product name: Histone H3(tri methyl K9) Monoclonal antibody
          Source: Mouse
          Applications: WB, IHC
          Species reactivity: Human,Rat,Mouse
          Recommended dilutions: WB 1:1,000-3,000 IP 1:200  IHC 1:200-500
          Immunogen: Synthetic Peptide
          Specificity: Human
          Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
          Clonality: Monoclonal
          Isotype: IgG1
          Concentration: 1mg/ml
          Observed band: 15kDa
          GeneID(Human): 8290
          Human Swiss-Prot No.: P68431
          Cellular localization: Nucleus,Chromosome
          Alternative Names: H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody
          Background: Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
          • Western blot analysis of Hela with EM1109 diluted at 1) 1:2,000 2) 1:5,000

          • Western blot analysis of 1) Rat Testis Tissue, 2) Raw264.7 with EM1109 diluted at 1:2000.

          • 1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1109 1:1,000 Secondary antibody: Goat anti-Mouse IgG(H+L) (S001) 1:10,000

          • Immunohistochemical analysis of paraffin-embedded Human Clolon using  Histone H3(tri methyl K9)(EM1109)Mouse mAb diluted at 1:500.