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          Catalog No. : ELK7392

          规格 价格
          48T ¥2240
          96T ¥3200


          Product name: 人干扰素诱导蛋白35(IFI35)酶联免疫吸附检测试剂盒
          Alternative Names: IFP35
          Reacted Species: Human
          Assay Type: Sandwich
          Sensitivity: 31 pg/mL
          Standard: 5000 pg/mL
          Range: 78.13-5000 pg/mL
          Sample type: Tissue homogenates, cell lysates and other biological fluids.
          Assay length: 4.5h
          Research Area: Cytokine;
          Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Inducible Protein 35(IFI35). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Inducible Protein 35(IFI35). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Inducible Protein 35(IFI35), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcitonin Interferon Inducible Protein 35(IFI35) in the samples is then determined by comparing the OD of the samples to the standard curve.