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              当前位置:首页产品展示ELISA试剂盒

              人LIM衰老细胞抗原样域蛋白1(LIMS1)酶联免疫吸附检测试剂盒

              Catalog No. : ELK7399

              规格 价格
              48T ¥2240
              96T ¥3200

              Overview

              Product name: 人LIM衰老细胞抗原样域蛋白1(LIMS1)酶联免疫吸附检测试剂盒
              Alternative Names: PINCH; PINCH1; Particularly interesting new Cys-His protein 1; Renal carcinoma antigen NY-REN-48
              Reacted Species: Human
              Assay Type: Sandwich
              Sensitivity: 0.056 ng/mL
              Standard: 10 ng/mL
              Range: 0.16-10 ng/mL
              Sample type: tissue homogenates, cell lysates and other biological fluids
              Assay length: 4.5h
              Research Area: Signal transduction;
              Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LIM And Senescent Cell Antigen Like Domains Protein 1(LIMS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to LIM And Senescent Cell Antigen Like Domains Protein 1(LIMS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LIM And Senescent Cell Antigen Like Domains Protein 1(LIMS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcitonin LIM And Senescent Cell Antigen Like Domains Protein 1(LIMS1) in the samples is then determined by comparing the OD of the samples to the standard curve.