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            人CD8b分子(CD8b)酶联免疫吸附检测试剂盒

            Catalog No. : ELK7411

            规格 价格
            48T ¥2240
            96T ¥3200

            Overview

            Product name: 人CD8b分子(CD8b)酶联免疫吸附检测试剂盒
            Alternative Names: CD8B1; CD8 Antigen,Beta Polypeptide 1; T-cell surface glycoprotein CD8 beta chain
            Reacted Species: Human
            Assay Type: Sandwich
            Sensitivity: 0.58 ng/mL
            Standard: 100 ng/mL
            Range: 1.57-100 ng/mL
            Sample type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
            Assay length: 4.5h
            Research Area: CD & Adhesion molecule;
            Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 8b(CD8b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 8b(CD8b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 8b(CD8b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcitonin Cluster Of Differentiation 8b(CD8b) in the samples is then determined by comparing the OD of the samples to the standard curve.