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        人白介素1θ(IL1q)酶联免疫吸附检测试剂盒

        Catalog No. : ELK7424

        规格 价格
        48T ¥2240
        96T ¥3200

        Overview

        Product name: 人白介素1θ(IL1q)酶联免疫吸附检测试剂盒
        Alternative Names: IL1F10; IL38; FKSG75; IL-1HY2; IL-1F10; FIL1-Theta; Interleukin-38; Interleukin-1 Receptor Antagonist FKSG75; Interleukin 1 Family, Member 10
        Reacted Species: Human
        Assay Type: Sandwich
        Sensitivity: 2.7 pg/mL
        Standard: 500 pg/mL
        Range: 7.82-500 pg/mL
        Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
        Assay length: 4.5h
        Research Area: Signal transduction;
        Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Theta(IL1q). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Theta(IL1q). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Theta(IL1q), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcitonin Interleukin 1 Theta(IL1q) in the samples is then determined by comparing the OD of the samples to the standard curve.