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      人细胞色素P450家族成员2C9(CYP2C9)酶联免疫吸附检测试剂盒

      Catalog No. : ELK7438

      规格 价格
      48T ¥2240
      96T ¥3200

      Overview

      Product name: 人细胞色素P450家族成员2C9(CYP2C9)酶联免疫吸附检测试剂盒
      Alternative Names: CYP2C10; P450IIC9; Cytochrome P450,Subfamily IIC(Mephenytoin 4-Hydroxylase)Polypeptide 9; S-mephenytoin 4-hydroxylase
      Reacted Species: Human
      Assay Type: Sandwich
      Sensitivity: 0.236 ng/mL
      Standard: 40 ng/mL
      Range: 0.63-40 ng/mL
      Sample type: Tissue homogenates, cell lysates and other biological fluids
      Assay length: 4.5h
      Research Area: Tumor immunity;
      Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 2C9(CYP2C9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 2C9(CYP2C9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 2C9(CYP2C9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcitonin Cytochrome P450 2C9(CYP2C9) in the samples is then determined by comparing the OD of the samples to the standard curve.