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            人自噬相关蛋白5(ATG5)酶联免疫吸附检测试剂盒

            Catalog No. : ELK7439

            规格 价格
            48T ¥2240
            96T ¥3200

            Overview

            Product name: 人自噬相关蛋白5(ATG5)酶联免疫吸附检测试剂盒
            Alternative Names: ASP; APG5; APG5L; hAPG5; Apoptosis-specific protein; Autophagy protein 5
            Reacted Species: Human
            Assay Type: Sandwich
            Sensitivity: 0.28 ng/mL
            Standard: 50 ng/mL
            Range: 0.79-50 ng/mL
            Sample type: serum, plasma, tissue homogenates and other biological fluids
            Assay length: 4.5h
            Research Area: Metabolic pathway;
            Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Autophagy Related Protein 5(ATG5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Autophagy Related Protein 5(ATG5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Autophagy Related Protein 5(ATG5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcitonin Autophagy Related Protein 5(ATG5) in the samples is then determined by comparing the OD of the samples to the standard curve.