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          人钠/钾离子转运ATP酶α2肽(ATP1a2)酶联免疫吸附检测试剂盒

          Catalog No. : ELK7441

          规格 价格
          48T ¥2240
          96T ¥3200

          Overview

          Product name: 人钠/钾离子转运ATP酶α2肽(ATP1a2)酶联免疫吸附检测试剂盒
          Alternative Names: FHM2; MHP2; Migraine,Hemiplegic 2; Sodium pump subunit alpha-2; Sodium/potassium-transporting ATPase subunit alpha-2
          Reacted Species: Human
          Assay Type: Sandwich
          Sensitivity: 0.103 ng/mL
          Standard: 20 ng/mL
          Range: 0.32-20 ng/mL
          Sample type: Tissue homogenates, cell lysates and other biological fluids
          Assay length: 4.5h
          Research Area: Signal transduction;
          Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Na+/K+ Transporting Alpha 2 Polypeptide(ATP1a2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Na+/K+ Transporting Alpha 2 Polypeptide(ATP1a2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Na+/K+ Transporting Alpha 2 Polypeptide(ATP1a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcitonin ATPase, Na+/K+ Transporting Alpha 2 Polypeptide(ATP1a2) in the samples is then determined by comparing the OD of the samples to the standard curve.